Article Navigation
- < Previous
- Next >
Journal Article
Get access
, Haemin Kim Department of Cell and Developmental Biology, BK21 Program and DRISeoul National UniversitySeoulKorea Search for other works by this author on: Oxford Academic Yong Deok Lee Department of Cell and Developmental Biology, BK21 Program and DRISeoul National UniversitySeoulKorea Search for other works by this author on: Oxford Academic Hyung Joon Kim Department of Oral PhysiologySchool of DentistryPusan National UniversityYangsanKorea Search for other works by this author on: Oxford Academic Zang Hee Lee Department of Cell and Developmental Biology, BK21 Program and DRISeoul National UniversitySeoulKorea Search for other works by this author on: Oxford Academic Hong‐Hee Kim Department of Cell and Developmental Biology, BK21 Program and DRISeoul National UniversitySeoulKorea Address correspondence to: Hong‐Hee Kim, PhD, Department of Cell and Developmental Biology, BK21 Program and Dental Research Institute, Seoul National University, 101 Daehak‐ro, Jongro‐gu, Seoul 03080, Korea. E‐mail: hhbkim@snu.ac.kr Search for other works by this author on: Oxford Academic
Journal of Bone and Mineral Research, Volume 32, Issue 2, 1 February 2017, Pages 397–406, https://doi.org/10.1002/jbmr.2974
Published:
19 August 2016
Article history
Received:
13 April 2016
Revision received:
08 August 2016
Accepted:
16 August 2016
Published:
19 August 2016
- Views
- Article contents
- Figures & tables
- Video
- Audio
- Supplementary Data
-
Cite
Cite
Haemin Kim, Yong Deok Lee, Hyung Joon Kim, Zang Hee Lee, Hong‐Hee Kim, SOD2 and Sirt3 Control Osteoclastogenesis by Regulating Mitochondrial ROS, Journal of Bone and Mineral Research, Volume 32, Issue 2, 1 February 2017, Pages 397–406, https://doi.org/10.1002/jbmr.2974
Close
Search
Close
Search
Advanced Search
Search Menu
ABSTRACT
Reactive oxygen species (ROS) are an indispensable element of cellular signal transduction in various cell types, including bone cells. In particular, osteoclasts (OCs), cells specialized for bone resorption, utilize ROS as second messengers during receptor activator of NF‐κB ligand (RANKL)‐induced differentiation and activation. In addition, because of the high energy demands of bone‐resorbing activity, OCs contain large amounts of mitochondria, the source of the majority of total ROS. In this study, we focused on the regulation of ROS generated from mitochondria during osteoclastogenesis. We observed that the level of mitochondrial superoxide dismutase 2 (SOD2), an enzyme responsible for reducing superoxide radicals in mitochondria, was increased by RANKL. siRNA‐mediated knockdown (KD) of SOD2 increased ROS levels and enhanced OC differentiation. Conversely, overexpression of SOD2 reduced osteoclastogenesis by decreasing ROS levels. Moreover, we found that NAD‐dependent deacetylase sirtuin 3 (Sirt3), an activator of SOD2 in mitochondria, was induced by RANKL. Sirt3‐targeted siRNA decreased SOD2 activity by reducing deacetylation of lysine 68 of SOD2, leading to increased osteoclastogenesis. Furthermore, in vivo KD of SOD2 or Sirt3 in ICR mouse calvariae decreased bone volume and increased OC surface, supporting the results of in vitro experiments. Taken together, our findings demonstrate for the first time to our knowledge that the regulation of mitochondrial ROS by SOD2 and Sirt3 plays an important role in fine‐tuning the OC differentiation program. © 2016 American Society for Bone and Mineral Research.
SOD2, SIRT3, OSTEOCLAST, RANKL, ROS
© 2017 American Society for Bone and Mineral Research
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
You do not currently have access to this article.
Download all slides
Sign in
Get help with access
-
American Society for Bone and Mineral Research members
Personal account
- Sign in with email/username & password
- Get email alerts
- Save searches
- Purchase content
- Activate your purchase/trial code
- Add your ORCID iD
Sign in Register
Institutional access
- Sign in with a library card
- Sign in with username/password
- Recommend to your librarian
Sign in through your institution
Sign in through your institution
Institutional account management
Sign in as administrator
Get help with access
Institutional access
Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:
IP based access
Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.
Sign in through your institution
Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.
- Click Sign in through your institution.
- Select your institution from the list provided, which will take you to your institution's website to sign in.
- When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.
Sign in with a library card
Enter your library card number to sign in. If you cannot sign in, please contact your librarian.
Society Members
Society member access to a journal is achieved in one of the following ways:
Sign in through society site
Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:
- Click Sign in through society site.
- When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If you do not have a society account or have forgotten your username or password, please contact your society.
Sign in using a personal account
Some societies use Oxford Academic personal accounts to provide access to their members. See below.
Personal account
A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.
Some societies use Oxford Academic personal accounts to provide access to their members.
Viewing your signed in accounts
Click the account icon in the top right to:
- View your signed in personal account and access account management features.
- View the institutional accounts that are providing access.
Signed in but can't access content
Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.
Institutional account management
For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.
Purchase
Subscription prices and ordering for this journal
Purchasing options for books and journals across Oxford Academic
Short-term Access
To purchase short-term access, please sign in to your personal account above.
Don't already have a personal account? Register
SOD2 and Sirt3 Control Osteoclastogenesis by Regulating Mitochondrial ROS - 24 Hours access
EUR €46.00
GBP £40.00
USD $49.00
Rental
This article is also available for rental through DeepDyve.
Advertisem*nt
Email alerts
Article activity alert
Advance article alerts
New issue alert
Receive exclusive offers and updates from Oxford Academic
Email alerts
Advance article alerts
New issue alert
Receive exclusive offers and updates from Oxford Academic
Related articles in
- Web of Science
- Google Scholar
Citing articles via
Google Scholar
-
Latest
-
Most Read
-
Most Cited
More from Oxford Academic
Clinical Medicine
Endocrinology and Diabetes
Medicine and Health
Neuroscience
Science and Mathematics
Books
Journals
Advertisem*nt
